Depth perception is primarily reliant on binocular vision, which involves using both eyes to create a three-dimensional understanding of space. When using only one eye, the brain attempts to compensate for the lack of depth cues, but this compensation is limited, especially in unfamiliar environments. Tests suggested in the discussion include covering one eye and gauging distances to objects, which illustrates the difficulty in accurately judging depth without binocular input. Familiarity with surroundings can aid in distance estimation, as prior knowledge allows the brain to fill in gaps. Various experiments, such as trying to touch fingertips or throw a ball, highlight the challenges of depth perception with one eye closed. The conversation also touches on the neurological aspects of vision, noting that depth perception can be affected by brain hemisphere communication, particularly in cases like corpus callosotomy. Overall, while some depth perception can be achieved with one eye through memory and head movement, it is significantly less accurate than with both eyes open.