4 parameter logistic model for fitting PCR data

requiem31
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I have a question about the logistic fit they used on this paper: http://online.liebertpub.com/doi/pdf/10.1089/cmb.2005.12.1047. Its PDF page 5, or journal page 1051. They define all the variables and then say x0 and b define the shape of the model. If I am fitting 30 cycles of PCR data how am I suppose to know what to use for x0 and b? -Thanks
 
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requiem31 said:
If I am fitting 30 cycles of PCR data how am I suppose to know what to use for x0 and b?
Use your fit results? Those results should be the unknown constants.
 

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