Chi square Dominant phenotype problem

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Homework Help Overview

The discussion revolves around a Chi-square analysis related to a genetic cross between Purple and White phenotypes. The original poster presents observed and expected values for the dominant and recessive traits, questioning the calculations and the resulting Chi-square value.

Discussion Character

  • Mixed

Approaches and Questions Raised

  • The original poster attempts to calculate the Chi-square value based on observed and expected counts for Purple and White phenotypes, expressing confusion over the expected values and the resulting Chi-square sum.
  • Some participants question the expected values for the White phenotype and suggest corrections based on the expected ratios from a monohybrid cross.
  • There is discussion about the assumptions regarding dominant and recessive traits in the context of the problem.

Discussion Status

Participants are actively engaging with the calculations and assumptions presented. Some guidance has been offered regarding the expected values, but there is no explicit consensus on the final calculations or interpretations of the Chi-square results.

Contextual Notes

There is a mention of the need to categorize the question appropriately within the forum, indicating a potential misunderstanding of the forum's structure. The discussion also reflects on the theoretical basis of the genetic cross being analyzed.

sonyab523
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What is the ChiSquare value for the dominant phentotype of the cross Purple versus White. Shouldn't the answer be 696 or 696.75??
Parental Phenotypes

Purple X WhiteF1 Phenotype

All PurpleF2 Phenotype

705 purple, 224 whiteI must be missing something:

Observed Purple=705

Observed White= 224

Total 929

Expected for Purple 705/929=.75

.75x929= 696.75 Purple Expected

Expected White 224/929=.24

.24 x 929= 222.96 White Expected
Second part of the question is

What is the Chi Square Sum (rounding to 3 significant figures) for Purple versus White cross. The answer is 0.391

But when I try to get this answer I use the formula (O-E)^2/E and get .09768 for Purple and .0048 for White which equals .1085
Third part of the question:

Using the following web site: (http://www.graphpad.com/quickcalcs/PValue1.cfm)
What is the P-value (rounding to 3 significant figures) for Purple versus White cross.
 
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Hello sonyab523,

Welcome to Physics Forum!

Btw, next time you might wish to put this sort of question in the
Physics Forums > Science Education > Homework & Coursework Questions > Other Sciences
category. 'Mostly just introductory physics here.

But anyway, I think I can help (I'm not an expert in genetics, but I'm not completely unfamiliar with it either):

sonyab523 said:
Observed Purple=705

Observed White= 224

Total 929

Expected for Purple 705/929=.75

.75x929= 696.75 Purple Expected

Expected White 224/929=.24

.24 x 929= 222.96 White Expected

Okay, here is where I believe you went wrong. The Expected White is 0.25 x 929 = 232.25.

In other words, 3/4 of this population is expected to be Purple, 1/4 White.

That makes the (O - E) for White -8.25.

Second part of the question is

What is the Chi Square Sum (rounding to 3 significant figures) for Purple versus White cross. The answer is 0.391

But when I try to get this answer I use the formula (O-E)^2/E and get .09768 for Purple and .0048 for White which equals .1085

Your Purple calculation is the same as mine. After you make the above correction for White (and recalculate), I think you should be good to move forward.
 
collinsmark said:
Hello sonyab523,

Welcome to Physics Forum!

Btw, next time you might wish to put this sort of question in the
Physics Forums > Science Education > Homework & Coursework Questions > Other Sciences
category. 'Mostly just introductory physics here.

But anyway, I think I can help (I'm not an expert in genetics, but I'm not completely unfamiliar with it either):



Okay, here is where I believe you went wrong. The Expected White is 0.25 x 929 = 232.25.

In other words, 3/4 of this population is expected to be Purple, 1/4 White.

That makes the (O - E) for White -8.25.



Your Purple calculation is the same as mine. After you make the above correction for White (and recalculate), I think you should be good to move forward.



I see I forgot to round. I have another question on a similar problem but I'll post start another thread in the right forum. Thank you.
 
sonyab523 said:
I see I forgot to round. I have another question on a similar problem but I'll post start another thread in the right forum. Thank you.

I was sort of under the impression that it's not a matter of rounding. What I assumed was that Purple and White represent a monohybrid cross between two heterozygotes. This is based more on theory, rather than on the measured statistics of the particular problem. I was assuming that the problem assumes one dominant phenotype (F1 phenotype), and one recessive (F2 phenotype). If my assumption was correct, it seems obvious here that Purple would be the dominant trait, call it "P", and White is the recessive trait, call it "w". Then there are only 4 possible combinations:

PP
Pw
wP
ww

But since Purple is dominant trait, any combination with a P in it will show the characteristics of purple. So PP, Pw, and wP all produce Purple characteristic -- that's an expected 3/4 of the population. Only the ww combination produces a White characteristic. 1/4 of the population.

[Edit] Or maybe to put it better, 3/4 and 1/4 of the total number of possible combinations.
 
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