# Difference between Working distance and object distance

• I
• Aymangh994
In summary, the conversation discusses the confusion between working distance and object distance of a lens, as well as the difference between image distance and back focal length. The question of how to calculate focal length, object distance, and image distance in order to enlarge an image by a factor of 6 is also raised. It is noted that while working distance and object distance may be the same for a perfectly thin lens, in reality they are measured from different points and are not equivalent.
Aymangh994
So I have a problem understanding the difference between working distance and the object distance of a lens.
For me it is also unclear what the difference between image distance and back focal length.
If I want to enlarge the image by 6. How can I calculate the focal length, object distance and image distance ?
Is the working distance the same as object distance ?

Typically only for a perfectly thin lens (which can be a useful approximation). The object distance is measured from the hypothetical lens center and the working distance from the actual front surface of the lens. In the real world dthay are not the same.

## What is the difference between working distance and object distance?

Working distance refers to the distance between the objective lens of a microscope and the specimen being observed. Object distance, on the other hand, refers to the distance between the specimen and the focal point of the objective lens. In simpler terms, working distance is the distance from the microscope to the specimen, while object distance is the distance from the specimen to the objective lens.

## Why is it important to know the working distance and object distance in microscopy?

Knowing the working distance and object distance is crucial in microscopy because it determines the magnification and clarity of the image. A shorter working distance allows for higher magnification, but can also result in a smaller field of view and decreased depth of field. On the other hand, a longer working distance provides a wider field of view and greater depth of field, but at a lower magnification.

## How can the working distance and object distance be adjusted in a microscope?

The working distance can be adjusted by moving the objective lens closer or further away from the specimen using the fine and coarse adjustment knobs. The object distance can be adjusted by moving the stage up or down using the stage adjustment knobs.

## What happens to the working distance and object distance when changing objectives on a microscope?

When changing objectives, both the working distance and object distance will change. Higher magnification objectives have a shorter working distance and require the specimen to be closer to the objective lens. This also means that the object distance will be shorter, resulting in a smaller field of view and decreased depth of field.

## Is there an ideal working distance or object distance in microscopy?

There is no one ideal working distance or object distance in microscopy, as it depends on the specific needs and objectives of the experiment. A shorter working distance may be preferred for higher magnification and resolution, while a longer working distance may be preferred for a wider field of view and greater depth of field. It is important to find the balance between these factors to achieve the desired results.

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