DsRNA inhibition

  • Thread starter Monique
  • Start date
  • #1
Monique
Staff Emeritus
Science Advisor
Gold Member
4,149
64

Main Question or Discussion Point

An interesting concept worth sharing: knocking a gene out in an organism.. how to do that.. now, dsRNA is used in the process. Eukaryotic organisms don’t have dsRNA (double stranded) only ssRNA. Some virusses though, DO have dsRNA. A eucaryotic organisms reacts to dsRNA by inhibiting the transcription of that particular RNA. But it turns out that ssRNA transcription of the particular kind is also inhibited! So if you’d like to knock out a gene in a model organism, inject it with dsRNA that codes for the gene. Every cell of the organism will react and knockout the fragment.

There must be some catches to the technique though because it sounds too simple :)
 

Answers and Replies

  • #2
Another God
Staff Emeritus
Gold Member
976
3
Originally posted by Monique

There must be some catches to the technique though because it sounds too simple :)
I am pretty sure that this doesn't work in every eukaryote. I know it was discovered in some flower, and they find it works in most plants I think.

Apparently it is an 'old' virus protection thing, and so many higher eukaryotes don't have the dsRNA protection system.
 
  • #3
Monique
Staff Emeritus
Science Advisor
Gold Member
4,149
64
Well, at least I know it works in C. elegans. It is true, I also heard it is an old mechanism.
 

Related Threads on DsRNA inhibition

Replies
12
Views
8K
Replies
17
Views
18K
  • Last Post
Replies
7
Views
9K
Replies
4
Views
4K
  • Last Post
Replies
1
Views
747
Replies
2
Views
2K
Replies
2
Views
2K
Replies
19
Views
7K
Top