Error measuring glucose optical sensor

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Discussion Overview

The discussion revolves around the challenges faced in measuring glucose concentrations using an optical sensor that employs phosphorescence and glucose oxidase technology. Participants are exploring the variability in sensor responses to different glucose concentrations and seeking solutions to improve measurement consistency.

Discussion Character

  • Exploratory
  • Technical explanation
  • Debate/contested

Main Points Raised

  • One participant describes the design of an optical sensor for glucose measurement but notes inconsistent changes in sensor response for varying glucose concentrations.
  • Another participant requests additional information regarding the sensor setup, including details about PBS, glucose concentration ranges, measurable variables, and noise levels.
  • A third participant emphasizes the need for clarity on what specific changes are being observed at different concentrations.
  • A later reply mentions that glucose oxidase may fluoresce under various light wavelengths and highlights the potential for inflection points in the response-versus-concentration graph, which could complicate readings.
  • Links to relevant articles are provided to assist in understanding the underlying principles and challenges associated with the sensor technology.

Areas of Agreement / Disagreement

Participants have not reached a consensus, as there are multiple competing views regarding the sensor's performance and the factors influencing measurement variability. The discussion remains unresolved, with ongoing requests for clarification and additional information.

Contextual Notes

Participants have noted limitations in the information provided, including missing details about the sensor's operational parameters and the specific nature of the observed changes in response to glucose concentrations.

niloofar
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Hello
We have designed an optical sensor to measure glucose.
But we do not have the same changes for different concentrations. What do we need to do to be able to observe different Lifetime concentrations?
In our sensor test, we used phosphorescence and glucose oxidase technology. The sensor is located in 3 ml of PBS and some glucose is added each time. We can see Lifetime changes, but these changes are sometimes incremental and sometimes decreasing for different concentrations.
Does anyone have a solution to our problem?
 
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We need much more information in order to help you.
1) What is PBS?
2) What range of glucose concentration?
3) What measurable variables change with glucose concentration?
4) What is the absolute magnitude of those variables?
5) What is the relative magnitude of those variables?
6) How are you measuring those variables?
7) What is the noise level?
8) What do mean by Lifetime concentrations and changes?
9) And a sketch of your sensor and test setup.
 
niloofar said:
But we do not have the same changes for different concentrations.
Same changes of what?. Different concentrations of what?
You need tobe much more explicit aaaand detailed here.
 
Way out of my field, but a Google search found the following:

When glucose oxidase is stimulated with various light wavelengths from UV to visible Blue, it will fluoresce somewhat. For some approaches, the hard part is the response-versus-concentration graph has an inflection point, so a reading can be mapped to at least two different concentrations. Also, some of the fluorescent lifetimes are in the ten's of nanoSeconds.

​

These articles may be of some use:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943336/
https://www.mdpi.com/2079-6374/10/8/86
https://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.415.2233&rep=rep1&type=pdf

(above, and many more, found with:
https://www.google.com/search?q=phosphorescence+and+glucose+oxidase)

Please let us know what you find and how your research is progressing.

Thanks,
Tom
 

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