Medical Error measuring glucose optical sensor

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An optical sensor designed to measure glucose levels using phosphorescence and glucose oxidase technology is facing challenges in detecting consistent lifetime changes across varying glucose concentrations. The sensor operates in a 3 ml PBS solution, but the observed lifetime changes are inconsistent, sometimes showing incremental increases and other times decreases. To address these issues, several clarifying questions were raised, including the specifics of PBS, the range of glucose concentrations tested, measurable variables affected by glucose, and the methods used for measurement. Additionally, the complexity of the response curve, which may have inflection points leading to ambiguous readings, was highlighted. Relevant articles were suggested to aid in understanding the relationship between fluorescence and glucose concentration. The discussion emphasizes the need for detailed information and clarity to troubleshoot the sensor's performance effectively.
niloofar
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Hello
We have designed an optical sensor to measure glucose.
But we do not have the same changes for different concentrations. What do we need to do to be able to observe different Lifetime concentrations?
In our sensor test, we used phosphorescence and glucose oxidase technology. The sensor is located in 3 ml of PBS and some glucose is added each time. We can see Lifetime changes, but these changes are sometimes incremental and sometimes decreasing for different concentrations.
Does anyone have a solution to our problem?
 
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We need much more information in order to help you.
1) What is PBS?
2) What range of glucose concentration?
3) What measurable variables change with glucose concentration?
4) What is the absolute magnitude of those variables?
5) What is the relative magnitude of those variables?
6) How are you measuring those variables?
7) What is the noise level?
8) What do mean by Lifetime concentrations and changes?
9) And a sketch of your sensor and test setup.
 
niloofar said:
But we do not have the same changes for different concentrations.
Same changes of what?. Different concentrations of what?
You need tobe much more explicit aaaand detailed here.
 
Way out of my field, but a Google search found the following:

When glucose oxidase is stimulated with various light wavelengths from UV to visible Blue, it will fluoresce somewhat. For some approaches, the hard part is the response-versus-concentration graph has an inflection point, so a reading can be mapped to at least two different concentrations. Also, some of the fluorescent lifetimes are in the ten's of nanoSeconds.

​

These articles may be of some use:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943336/
https://www.mdpi.com/2079-6374/10/8/86
https://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.415.2233&rep=rep1&type=pdf

(above, and many more, found with:
https://www.google.com/search?q=phosphorescence+and+glucose+oxidase)

Please let us know what you find and how your research is progressing.

Thanks,
Tom
 
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