Anybody can help for calculation of the heat of the following reaction? Some background: Ligand X bind to protein M, the product is MX. Binding affinity of X to M is K = [MX]/([M]*[X]). [MX], [M], [X] are the concentrations of each stuff after the reaction finished. The ratio of MX to total M is θ = [MX]/([M]+[MX]). ΔH is the molar heat of M, V is the volume of reaction. Mt and Xt is the concentration of total M and X at the beginning of the reaction. So, for this reaction the heat Q = θ*Mt*ΔH*V My Question is: The beginning products: Xt= 50 uM, Mt=400 uM, also some [MX] = 4 um, ( Mt is the total concentration of M, including the M in MX), V=200 ul, K= 100000 M-1, and ΔH =-4000 How to calculate the heat Q for above reaction? Tons of thanks!!