Net Ionic Lab Question (the lab procedure)

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Discussion Overview

The discussion revolves around the procedural instructions for a net ionic lab, specifically focusing on how to properly set up and execute the experiment involving multiple solutions and their reactions to form precipitates. The scope includes clarifications on the lab procedure and the arrangement of reagents in a well plate.

Discussion Character

  • Homework-related, Technical explanation, Debate/contested

Main Points Raised

  • One participant expresses confusion about the instruction to place drops of reagent 1 in "all walls in column 1 and row A," questioning whether this means to use only one well.
  • Another participant clarifies that it means to place drops in all wells of column 1 and all wells of row A, suggesting that this is necessary for proper mixing.
  • A third participant agrees with the interpretation that all wells in column 1 and row A should be filled, emphasizing the need for mixing to observe reactions.
  • Further discussion raises the concern about the order of adding drops and whether all drops should be added before observing precipitates, indicating a potential misunderstanding of the experimental design.
  • One participant inquires about the instructions for reagents 5 and 6, indicating a need for further clarification on those specific reagents.
  • Another participant suggests drawing a plan of the grids to visualize the setup, proposing that each combination of reagents is tested twice.

Areas of Agreement / Disagreement

Participants generally agree on the interpretation of the instructions regarding the placement of reagent drops, but there is some uncertainty about the overall experimental design and the specifics of mixing reagents. The discussion remains unresolved regarding the exact procedure for reagents 5 and 6.

Contextual Notes

There are limitations in the clarity of the instructions provided for the lab, particularly regarding the mixing of reagents and the observation of precipitates. Participants express confusion about the sequence of actions and the specific combinations being tested.

lc99
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Homework Statement


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Homework Equations



n/a

The Attempt at a Solution



I'm kinda confused on the instructions of this net ionic lab. For this lab, I am suppose to find out how several solutions (6 of them) react with each other to form precipitates.

We use some systematic way to test these by putting them in a 24 plate well. But, i am confused because if how they want me to put the drops of 'reagents' in each well.

For instance it says, reagent 1: all walls in column 1 and row A. Doesn't this mean that i only put the drops of reagent 1 in ONE well? Since column 1 and row A is just 1 well?
 

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No, all wells in column 1 and all wells in row A. Otherwise whole experiment doesn't make sense.
 
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I assume it means "all wells in column 1 and all wells in row A", i.e. all wells that are in either column 1 or row A (or both). Otherwise you won't get any mixing.

Beat me to it!
 
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Borek said:
No, all wells in column 1 and all wells in row A. Otherwise whole experiment doesn't make sense.
So I do all the drops for all the wells before observing the precipitates and taking down notes? Right? Cause, the order of the droppings wouldn't make sense if i was trying to observed and record the precipitates

Also, in the second image.. it is unclear on what i am mixing .. It says A1,B1 for reagent 1. What exactly am i mixing reagent 1 with then?
 
What does it say for reagents 5 and 6?
 
mjc123 said:
What does it say for reagents 5 and 6?
Well. It does mix them together but I am not picturing how this is testing the solutions again or the point of this
 
See note to no. 12. I suggest you draw a plan of the two grids and note what you put in each well. You should find you test each combination twice.
 

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