Net Ionic Lab Question (the lab procedure)

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lc99
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Homework Statement


Figure 1 labeling a 24-well plate: a 4 by 6 grid of wells with rows A to D and columns 1 to 6, with a 'Well' arrow pointing to well A-1; the reagent assignment list beneath it is printed.

Photo of a lab procedure page, step 12, listing reagent combinations A1-A6 and B1-B6 to test in a well plate, with a note to repeat tests and check results.

Homework Equations



n/a

The Attempt at a Solution



I'm kinda confused on the instructions of this net ionic lab. For this lab, I am suppose to find out how several solutions (6 of them) react with each other to form precipitates.

We use some systematic way to test these by putting them in a 24 plate well. But, i am confused because if how they want me to put the drops of 'reagents' in each well.

For instance it says, reagent 1: all walls in column 1 and row A. Doesn't this mean that i only put the drops of reagent 1 in ONE well? Since column 1 and row A is just 1 well?
 
Attachments
  • Labeled diagram of a 24-well plate with rows A to D and columns 1 to 6, with a 'Well' arrow pointing to row A, followed by the reagent placement rules listed below it.
    upload_2018-2-28_11-39-39.png
    66.8 KB · Views: 1,343
  • Figure 1 labeling a 24-well plate: a 4 by 6 grid of wells with rows A to D and columns 1 to 6, with a 'Well' arrow pointing to well A-1; the reagent assignment list beneath it is printed.
    upload_2018-2-28_11-39-53.png
    66.8 KB · Views: 1,313
  • Photo of a lab procedure page, step 12, listing reagent combinations A1-A6 and B1-B6 to test in a well plate, with a note to repeat tests and check results.
    upload_2018-2-28_11-40-12.png
    28.4 KB · Views: 1,451
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No, all wells in column 1 and all wells in row A. Otherwise whole experiment doesn't make sense.
 
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I assume it means "all wells in column 1 and all wells in row A", i.e. all wells that are in either column 1 or row A (or both). Otherwise you won't get any mixing.

Beat me to it!
 
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Borek said:
No, all wells in column 1 and all wells in row A. Otherwise whole experiment doesn't make sense.
So I do all the drops for all the wells before observing the precipitates and taking down notes? Right? Cause, the order of the droppings wouldn't make sense if i was trying to observed and record the precipitates

Also, in the second image.. it is unclear on what i am mixing .. It says A1,B1 for reagent 1. What exactly am i mixing reagent 1 with then?
 
mjc123 said:
What does it say for reagents 5 and 6?
Well. It does mix them together but I am not picturing how this is testing the solutions again or the point of this
 
See note to no. 12. I suggest you draw a plan of the two grids and note what you put in each well. You should find you test each combination twice.