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I have already obtained a graph, and I used a program called "Graphpad Prism" to analyse the data usin nonlinear regression (3rd degree polynomial). I have got all the parameters like sy.x, degrees of freedom... But how do I use these parameters to analyse whether my results are reliable, and by varying enzyme concentration results in an increase in rate?

I have also considered Spearman's rank coefficient but this is only for linear relationships. What else can I do to determine whether my results show regression?

Thank you very much.