- #1
TytoAlba95
- 132
- 19
I was reading RAPD kit manual by himedia and couldn't understand why the two primers should be at close proximity and in opposite orientation to actually amplify DNA.
'In RAPD analysis, for PCR to occur: a) the primers must anneal in a particular orientation (such that they point towards each other) and, b) they must anneal within a reasonable distance of one another. Successful primer pairs produce different banding profiles of PCR products between individuals, strains, or species when analyzed by gel electrophoresis.'
'In RAPD analysis, for PCR to occur: a) the primers must anneal in a particular orientation (such that they point towards each other) and, b) they must anneal within a reasonable distance of one another. Successful primer pairs produce different banding profiles of PCR products between individuals, strains, or species when analyzed by gel electrophoresis.'
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