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quietviolence
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Homework Statement
Escherichia coli data:
molecular weight = 74000g/mol
specific volume = 0.737cm3/g
temperature = 293 K
In a dilute aqueous buffer (0.02M potassium phosphate) at 293.75 K
speed of rotation = 56050 rpm
gave the following results:
Time (min)...x1/2
0.....5.9
20...6
40...6.1
60...6.2
80...6.3
100.....6.4
120.... 6.5
140.....6.6
Homework Equations
This is the equation given to us:
M=(RTs)/(D(1-([tex]\rho[/tex] f *V o))
The Attempt at a Solution
I used an R value of 8.31m2kgmol-1s-2K-1
I rearranged the equation to equal s
However, I got stuck at [tex]\rho[/tex]f*Vo. Using the [tex]\rho[/tex] and V given in the question results in a division by 0.
Could someone explain to me how I find [tex]\rho[/tex] f and V o? I don't need anyone to solve the question, I would like to do that on my own.