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Sedimentation coefficient

  1. Nov 16, 2009 #1
    1. The problem statement, all variables and given/known data
    Escherichia coli data:
    molecular weight = 74000g/mol
    specific volume = 0.737cm3/g
    temperature = 293 K

    In a dilute aqueous buffer (0.02M potassium phosphate) at 293.75 K
    speed of rotation = 56050 rpm

    gave the following results:

    Time (min)....x1/2
    0..................5.9
    20................6
    40................6.1
    60................6.2
    80................6.3
    100..............6.4
    120............. 6.5
    140..............6.6


    2. Relevant equations
    This is the equation given to us:

    M=(RTs)/(D(1-([tex]\rho[/tex] f *V o))


    3. The attempt at a solution
    I used an R value of 8.31m2kgmol-1s-2K-1

    I rearranged the equation to equal s

    However, I got stuck at [tex]\rho[/tex]f*Vo. Using the [tex]\rho[/tex] and V given in the question results in a division by 0.

    Could someone explain to me how I find [tex]\rho[/tex] f and V o? I don't need anyone to solve the question, I would like to do that on my own.
     
  2. jcsd
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