What Does Edman Degradation Reveal About Peptide Sequence?

AI Thread Summary
The discussion revolves around determining the structure of an unknown pentapeptide E based on its amino acid composition and the results of Edman degradation and carboxypeptidase treatment. The peptide consists of glycine, tryptophan, proline, valine, and alanine, with proline being released first by carboxypeptidase. Edman degradation yielded three derivatives, indicating the sequence of amino acids, with tryptophan, glycine, and valine appearing in that order. Clarifications are sought regarding the specific type of carboxypeptidase used and the meaning of "separate Edman degradations." The conversation highlights the importance of understanding enzymatic specificity in peptide sequencing.
chem_monkey
Messages
3
Reaction score
0
I'm looking at a (uni) past paper and it has one of those typical Qs - a molecular weight determination has shown that an unknown peptide E is a pentapeptide and that it contains the amino acids glycine, tryptophan, proline, valine and alanine. Treatment of pentapeptide E with carboxypeptidase gives proline as the first free amino acid released. Separate Edman degradations provided the following derivatives: and it shows three compounds, with the first containing tryptophan, second just glycine, third valine.
Based upon this analysis, draw the structure of the original pentapeptide and explain how you have arrived at this structure. Show mechanistic detail where appropriate (15 marks).

Anyone know?
 
Physics news on Phys.org
Which flavor of Carboxypeptidase are you using? "Separate Edman degradations..." refers to what?
 
chemisttree said:
Which flavor of Carboxypeptidase are you using? "Separate Edman degradations..." refers to what?

what flavor?
 

Thread 'Prove that evolution is constant (Provide at least two arguments to support your position)'

I don't get how to argue it. i can prove: evolution is the ability to adapt, whether it's progression or regression from some point of view, so if evolution is not constant then animal generations couldn`t stay alive for a big amount of time because when climate is changing this generations die. but they dont. so evolution is constant. but its not an argument, right? how to fing arguments when i only prove it.. analytically, i guess it called that (this is indirectly related to biology, im...
View full post »

Similar threads

What Is Alpha-Complementation in Beta-Galactosidase?
Replies
1
Views
3K
How Does the Bdellovibrio Genome Advance Antimicrobial Research?
Replies
5
Views
3K
Could New Materials Propel China's Industry to Bill Gates-Level Success?
Replies
4
Views
10K

Hot Threads

Recent Insights

Back
Top