Chromosomes can be accurately counted during the metaphase stage of mitosis due to their alignment along the metaphase plate, which makes them easily visible and distinguishable. In this phase, chromosomes are condensed and organized, allowing for clear identification and counting. Other phases, such as prophase and anaphase, present challenges due to chromosomes being less organized or in motion, making counting difficult. Karyotype analysis often utilizes metaphase images for this reason. Understanding the structural arrangement of chromosomes in metaphase facilitates accurate chromosome counting.