Hi Happiness,
I will tackle your three questions separately.
1) Your question was if an isometry would preserve ##n##-volume. The answer is yes, it would, but we must be a bit careful. If ##A \subset \mathbb{R}^n## then we define the volume of ##A## to be $$v(A) = \int_A 1$$. It can be shown (see Munkres' "Analysis on Manifolds", page 176) that a map ##h: \mathbb{R}^n \rightarrow \mathbb{R}^n## is an isometry if and only if ##h(x) = Ax + p## where ##A## is an orthogonal matrix. This is a pretty intuitive fact. We can now prove that an isometry preserves volume: We know that ##Dh(x) = A##. It is not hard to see that ##h## is a diffeomorphism. Thus by change of variables theorem, $$\begin{align*} v(h(A)) &= \int_{h(A)} 1 \\ &= \int_{A} \lvert \det(A) \rvert \\ &= v(A) \end{align*}$$ Thus ##h## preserves ##n##-volume.2) Your question was why doesn't a bijective map preserve point-wise distance. The answer to this is that it is just a feature of bijections. Think of the bijection that sends ##(0,1)## onto ##(-2,2)##. If you picture ##(0,1)## as a line segment that is made out of a stretchy material (think of a rubber band), then the bijection stretches it out until it is ##(-2,2)##. Even though this is a bijection, pointwise distance has not been preserved.3) Your question was must a region-wise area-preserving map be injective. If by this, you meant, "given a set ##A \subset \mathbb{R}^n##, if a map preserves its area, must that map be injective?" The answer to that question would be no. PeroK answered it above. Now if you meant, "must a map which preserves the volume of ALL regions (which have a well-defined volume) be injective?". The answer to that is, I don't know. I think that the answer may be no, it does not need to be injective. I am thinking that you could possibly have a map which sends each region to itself, plus a fixed set of measure zero. Such a map would not be injective and would preserve volume since zero sets do not affect volume. I would have to think about this a bit more to be certain though.