Why do smaller particles travel faster in gel electrophoresis?

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In gel electrophoresis, smaller particles travel faster due to their lower mass and greater mobility in the gel matrix. While all particles experience the same electromotive force, their acceleration differs based on size, as described by F=ma. Larger particles face more resistance from the gel, which counteracts the applied force, resulting in slower movement. The steady-state condition indicates that the electrophoretic force is balanced by a frictional force that increases with particle size. Understanding these dynamics is crucial for interpreting electrophoretic results.
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Hello guys,

Sorry it is electrophoresis

In electrophoresis is a similar electromotive force applied to all particles or are all particles accelerated at the same rate?

What I mean is let's say if every particle gets 10N.

F=ma and bigger particle would travel slower. So can't I include this in the answer. If they are only accelerated at the same rate I can understand how different particles would have different resistances?

So is the answer F=ma?

Thank you! :smile:
 
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Think about the steady state situation where each particle is traveling at a constant velocity. The electrophoretic force must be opposed by another counteracting force. What is this force, and how might it depend on particle size?
 
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