Using FITC Fluorescein for Microscale Experiment

[kthouz]

Hello,
I am a physics student and I am trying to set up a microscale chemical experiment that involves Sulfuric Acid, Feroin, Molonic Acid, Sodium Bromade and water. I want to study the physical behavior of this reaction as I change the concentration of different components such as Sulfuric Acid. So, I want to encode the Surfuric acid with FITC fluorescein such that I can trace the amount of the fluorescence and hence quantify the concentration in Sulfuric Acid. I have been doing this without that fluorescence but my results are being crappy and I think this may be due to pipeting inaccuracy. By adding that dye, I will be able to interprete very accurately my results.
My worries is I don't know what kind of chemical reactions can go on between fluorescein and those other chemical components. Can anybody help me in any way?
Thank you

 

[Navin A S]

!</code>It is possible to use FITC fluorescein to trace the amount of sulfuric acid in your experiment. However, you should be aware of potential interactions between the FITC fluorescein and the other components of your reaction. For example, FITC fluorescein can react with Sulfuric Acid to form a fluorescent complex, which could interfere with your results. Additionally, FITC fluorescein can also become degraded or adsorbed onto surfaces, which could reduce its effectiveness as a tracer. It is important to do some research into the safety and reactivity of FITC fluorescein before using it in an experiment. Additionally, you should consider using controls to make sure that the FITC fluorescein is not interfering with your results.