What are the Differences Between Transmission and Scanning Electron Microscopes?

In summary, the transmission electron microscope (TEM) was the original form of electron microscopy and has a better resolution than the scanning electron microscope (SEM). The main difference between the two is the type of electrons detected, with TEM directly detecting transmitted electrons and SEM detecting backscattered and secondary electrons. This results in different sample preparation methods and capabilities, with SEM being able to quickly collect surface information over a larger area. Additionally, scanning TEM (STEM) was developed to incorporate a rastering capability for larger area coverage. Lastly, it should be noted that scanning tunneling microscopy (STM) uses quantum tunneling electrons, not SEM.
  • #1
kevin86
20
0
Is transmission electron microscope the original EM, and Scanning the better resolution one. What is the main difference between the two
 
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  • #2
They both use electrons.
Other than that, I can't think of anything that's the same.
I suppose you could say Scanning uses quantum tunneling electrons while the TM uses clasical electrons.
 
  • #3
kevin86 said:
Is transmission electron microscope the original EM, and Scanning the better resolution one. What is the main difference between the two
TEM was the first form of electron microscopy (the invention won Ruska his Nobel Prize). It also has a better resolution than SEM.

The main difference between the two is what gets detected. In SEM, you detect backscattered and secondary electrons (these are electrons emitted due to collisions from the impinging beam and other secondary phenomena), while in TEM you directly detect the transmitted electrons. Consequently, it requires a very different sample preparation for the two techniques. Notably, TEM samples need to be extremely carefully thinned to ensure a reasonable signal to noise ratio. Sample preparation is relatively, a walk in the park for SEM samples. Also, with the SEM, you can raster the e-beam across the area of the sample and (reasonably quickly) collect surface information about a fairly large area. The inability to do this with the traditional TEM was overcome somewhat by incorporating a rastering capability into what then became the STEM (Scanning TEM). Naturally, there's a trade off between resolution, and areal coverage.

NoTime said:
I suppose you could say Scanning uses quantum tunneling electrons while the TM uses clasical electrons.
That's STM you have in mind, not SEM.
 

Related to What are the Differences Between Transmission and Scanning Electron Microscopes?

What is an electron microscope?

An electron microscope is a scientific instrument that uses a beam of accelerated electrons to magnify objects at a much higher resolution than a traditional light microscope.

How does an electron microscope work?

An electron microscope works by using a beam of electrons instead of light to magnify an object. The electrons are focused and accelerated towards the object, creating a highly detailed image that can be viewed on a screen or photographed.

What are the advantages of using an electron microscope?

The main advantage of using an electron microscope is its ability to produce images with a much higher resolution than a traditional light microscope. This allows scientists to see smaller details and structures in the object being viewed.

What can be observed using an electron microscope?

An electron microscope can be used to observe a wide range of objects, from cells and tissues to microorganisms and nanoparticles. It is also commonly used in materials science to study the structure and composition of various materials.

Are there any limitations of using an electron microscope?

While an electron microscope has many advantages, it also has some limitations. It can only be used to observe non-living objects, and the preparation of samples for electron microscopy can be time-consuming and complex. Additionally, electron microscopes are expensive and require specialized training to operate.

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