Dear allI work with a spectrophotometer

[atish]

dear all
I work with a spectrophotometer "Carry 17D". below 300nm wavelenght the transition spectrom have lots of flactuation. how to solve the problem and how to provide a clear spectrom?
regards

 

[Claude Bile]

Often spectrometers will compute a baseline and then normalise spectra to that baseline. If the signal to noise ratio drops at shorter wavelengths, then normalised spectra will become progressively noisier at the shorter wavelengths.

To resolve this, you need either a brighter source, a better detector, or optics/gratings that have lower losses below 300 nm.

Measuring lots of spectra and averaging them can also reduce the noise, but this approach is time-consuming.

Claude.