Raman spectroscopy Definition and 33 Threads

I am attempting to revive an old Raman spectrometer that had a few components stop working. I was left with a functioning probe head, laser system, and some optical components. Currently, I have purchased a new CCD spectrometer and a few optical components to replace those that were specific to...

Hello everyone! I don't know much about quantum physics. I'm an amator, but I want to expand and deepen my knowledge in quantum. So, I decided on the Quantum Gravity Gradiometer to be the subject of my supervised personal initiative work. But I'm currently stuck with many things among which are...

I know that for the vibrational Raman spectrum, the energy levels are given by- E(n) = ℏω(n+1/2) - χℏω(n+1/2)^2 But I'm not getting what does it meant by the Raman lines are observed at given values of wavelengths and the fundamental vibrational frequency. I equated the E(0) with the energy...

I don't know whether it is an energy of a photon emitted by a deexciting molecule, or if it is an energy of laser's photons. Here is an example of such spectrum: For example, that value of wavenumber ##3000\, \mathrm {cm^{-1}}## is an energy of an emitted photon or a photon from laser? And that...

Raman spectroscopy involves Illuminating a target with a laser Beam. This produces Raleigh scattering. Does The releigh scattering have the same frequency as the laser beam? So use an optical filter to block the same frequency as the laser?

Can raman spectroscopy identify any substance or are there certain Substances raman spectroscopy cannot identify?

Hello everyone, I need some confirmation on something: As far as I understood, the raman spectroscopy measures the inelastic scattering of a photon in a medium through the absorption or the emission of a phonon in the medium. The energy and the momentum is conserved...

Hello, Recently I had a problem in the analysis of a raw material called Propranolol HCl, when performing Raman identification for the specific batch the obtained spectrum does not generate signal and, consequently, identity of the material. By testing other techniques like infrared and NMR, I...

I would like to know how to extract the instrumental broadening effect in Raman spectrometer using solid angle of the objectives, slit width or spectrometer length. I am calculation the FWHM in Raman peaks and I would like to know the effect of instrumental broadening on Raman line width and how...

Hi, The following is the typical raman spectra of water. In their normal form, water molecules maintain a simple configuration: an oxygen atom is in the center and two hydrogen atoms are on the two sides symmetrically. Under ambient conditions, water molecules within liquid water give rise to...

This may be a completely terrible question, but does someone have an idea on the detection capabilities for raman spectroscopy of a bulk sample like human tissue (hair, blood, skin, anything)? I thought it might be fun to see if it were possible to use raman to identify exposures to chemicals...

Hello! How are you? I am working in the photonics Lab in my university and I was wondering if has anyone ever tried to controll the horiba spectrometer T64000 with Labview. I know there is a way to do that with activx container. I was just wondering if that works. If it does, do you have any...

Homework Statement Sulfur hexafluoride is a centrosymmetric molecule with four infrared inactive vibrational modes: 346 cm-1 , 524 cm-1 , 643 cm-1 and 775 cm-1 The Raman spectrum of a liquid sample of SF6 (held at elevated pressures) was measured with a laser that has a centre frequency of...

Why do peaks of Stokes higher than peaks of anti-Stokes in graph of absorbance unit against wave number?

Hi everyone, I'm little bit confuse about non destructive techniques such as XRD (X-ray Diffraction) and Raman spectroscopy, I know that both techniques can determine the crystal structure of the certain materials, and also, I know that XRD is working with the principle of emission of x-ray...

What are the axes(horizontal & vertical axes) in a Raman spectrum?

Dear friends I have a question regarding to polarized Raman spectroscopy. When we are talking about parallel and perpendicular electric field of the laser, is the magic angle 90° or 54.7°? I'd be grateful if you could answer me as soon as possible. Best wishes Kasra

Homework Statement The Raman spectrum of ethene, obtained using 488.0nm radiation from a laser, shows an intense line at 540.0nm. Calculate the wavenumber for this vibration I know that you get stokes, anti-stokes and rayleigh scattering. Not sure how to calculate the wavenumber though ...

Hello, I am currently in an analytical chemistry course where I am required to write a report on Raman Spectroscopy. The typical description of Raman Spectroscopy is the excitation of an electron to a "virtual" state, whereupon it can relax to a non zero vibrational level in its ground...

hey guys, i hope you can help. my task is to analyse data of raman spectroscopy. therefor i have to deconvolute it. that means the data must have been convoluted somewhere. is it true that the raw data which i receive is convoluted already? or is it common to convolute the data "active"...

I'm currently working on a lab that is exploring the Raman effect. One of the suggested exercises was to record the raman spectrum (from a mercury lamp through CCl4) around the rayleigh peaks at 435.8 nm and 404.7 nm. For the former, my results were fairly consistent with what I expected in that...

Tell me what do the peaks in the graph show which is b/w Intensity and wave number in raman spectroscopy ? Are the peaks due to transition from a grnd state to an exicted state or vice versa , low energy are stokes and high energy are the anti stokes one

When you are setting up a spectral acquisition with a raman microscope, what does the exposure time mean? I would expect this to mean the amount of time the sample is exposed to the laser, but it doesn't seem like that can be true if it takes around 20 minutes to acquire the spectrum with a 2...

Can one monitor dynamics of a specific protein domain (helix, strand etc) by Raman Sp? If one labels a specific residue with an isotope, like in NMR, can one use that to monitor the dynamics of the region that encompasses this residue?

Please can anyone suggest a good book on raman spectroscopy. cheers.

Hi, For someone my question could be very simple but I couldn't find a well explain paper that explain this to me. In a Raman spectrometer the stokes and anti-stokes wavelength will always be grater than the laser wavelength used?. I have this confusion because I saw that Raman...

What are some simple graphs that can be created (in Matlab) relating to Raman Spectroscopy or just basic equations that can somehow be related to it? So far all I've done is graph E=E_0 cos (wt + 1/2 αt^2). And any suggestions of things I should read up on about the topic and understand...

Hello Group! I'm working on analyzing Raman spectra of proteins. I'm particularly interested in learning more about the signal processing of the amide I band. If anyone has any experience with Peakfit program or Origin, Savitsky-Golay smoothing, or amide I deconvolution for secondary...

I'm a freshman physics major and I'm doing summer research on Raman Spectroscopy. I've read up on it a bit but I was wondering if there are any resources that would help me better understand or at least get a general idea of what this is.

Raman spectroscopy of protein please help Hey any Raman experts out there? Im doing Raman spectroscopy of a protein called c-reactive protein (in aquous solution) using 532nm 10mW and the protein placed in a nmr tube. I can nicely get a spectrum of benzene and cyclohexane, but when I am doing...

I read in a journal article that in raman spectroscopy "it is well known that signal yield increases with shorter wavelength". Can anyone please explain why? I would've thought longer wavelengths = shorter frequencies = higher susceptibilities?

Raman spectroscopy is "Single molecule identification" Hi , I heard that the speciality of Raman spectroscopy is "Single molecule identification", it'd be really great if somebody can tell me HOW! because its surely going to be a question in my exam! Thanks in advance.

raman spectroscopy! Hi, can anybody please tell what is the speciality of Raman spectroscopy and how it differs from other techniques?? does it have any uniqueness? more info is welcomed! thanks in advance.