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488nm LED's?

  1. Apr 26, 2015 #1
    Why cant I find any LED's at 488nm? Im trying to find a cheaper light source than the traditional argon ion laser for a possible fluorescence activated cell sorter. See link below for schematic. Does anybody know a good retail site for lights in this spectrum I cant find good results with google.

    http://thebigone.stanford.edu/papers/Fu%20nature%20america.pdf [Broken]
     
    Last edited by a moderator: May 7, 2017
  2. jcsd
  3. Apr 26, 2015 #2

    phinds

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    How well focused does it have to be? That is, if it has a significant amount at 488 is it OK for it to have quite a bit in the surrounding region of the spectrum and if so, how far away from 488 is OK? There are inexpensive LED sources that show a range that includes 488.
     
    Last edited by a moderator: May 7, 2017
  4. Apr 26, 2015 #3
    I have no idea, i mean i bet i can filter out the unwanted parts of the spectrum anyways but really I think for biofluorescence it shouldnt take much power. When i think of a laser and high power, all that does is burn whatever it's pointed at. Not trying to burn the cultures. I would much rather try out the cheap version than spend a bunch of money on something i dont really need. Can you link me what you see at 488?
     
  5. Apr 27, 2015 #4

    phinds

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    Hm ... I was thinking UV spectrum at 388, not visible at 488. I can't see why you would want 488 if what you are looking for is fluorescence. To get 488 just buy a $4 "black light" bulb at a hardware store (but I don't think you'll get any fluorescence off of what you shine it on; at least I know that's true of wood)
     
  6. Apr 27, 2015 #5

    Andy Resnick

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    You aren't looking very hard (keeping in mind that Ar lasers are rather expensive):

    https://www.thorlabs.com/thorproduct.cfm?partnumber=NDS1113T
    http://ldselection.com/product/1105
    http://www.laserglow.com/J48
    http://lasers.coherent.com/lasers/488-nm-diode-laser

    Note, for live cell applications the 'critical link' is the detector- you want to illuminate with as low an intensity as possible, so you need a very sensitive detector.
     
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