Amino acid biosynthesis from CAC intermediates

[Jikx]

Hello,

I'm having a bit of trouble with an experiment. It involves converting furamate and citrate into amino-acids using a transaminase. We are using a liver homogenase, with the amino-donor being alanine.

However, whatever resource i use, furamate/citrate is not converted into an animo-acid!

Any suggestions? THANKS!

(my experiment failed, but I still to write up the discussion!)

 

[Jikx]

I worked it out.. kind of.

Citrate is converted to oxaloacetate (via CAC), then undergoes a transamination reaction to Aspartate.

Furamate is converted to a-ketoglutarate (also via CAC), then becomes Glutamate.

Its just a curse with me... always ignore the simplest answer because its too simple.

 

[ravenprp]

Hello,

I'm sorry to hear that your experiment did not go as planned. Amino acid biosynthesis from CAC intermediates is a complex process and can be affected by many factors. It is possible that there were some issues with the experimental setup or the conditions in which the reactions were carried out. It would be helpful to provide more details about the experiment, such as the specific protocol and conditions used, in order to better understand the issue and provide suggestions for improvement. Additionally, it may be beneficial to consult with a mentor or colleague who has experience with similar experiments to troubleshoot and identify potential sources of error. It is important to thoroughly discuss any unexpected results and potential sources of error in your write-up to accurately reflect the findings of the experiment. I wish you the best of luck in your future experiments.