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Amino acid biosynthesis from CAC intermediates

  1. Sep 19, 2004 #1

    I'm having a bit of trouble with an experiment. It involves converting furamate and citrate into amino-acids using a transaminase. We are using a liver homogenase, with the amino-donor being alanine.

    However, whatever resource i use, furamate/citrate is not converted into an animo-acid!

    Any suggestions? THANKS!

    (my experiment failed, but I still to write up the discussion!)
  2. jcsd
  3. Sep 21, 2004 #2
    I worked it out.. kind of.

    Citrate is converted to oxaloacetate (via CAC), then undergoes a transamination reaction to Aspartate.

    Furamate is converted to a-ketoglutarate (also via CAC), then becomes Glutamate.

    Its just a curse with me... always ignore the simplest answer because its too simple.
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