Calculating Centrifugation Distance and Acceleration for Blood Plasma Separation

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SUMMARY

The discussion focuses on calculating the centrifugation distance and acceleration required for effective blood plasma separation using a centrifuge. It specifies that an acceleration of 2000g is necessary, with the centrifuge operating at a frequency of 3480 rpm. The test tubes used are 14.3 cm long and tilted at a 45° angle. The calculations involve using the formula for centripetal acceleration, a = v²/r, and the relationship between angular velocity and radius to determine the distance from the rotation axis and the accelerations at both ends of the test tube.

PREREQUISITES
  • Understanding of centripetal acceleration and its formula, a = v²/r
  • Familiarity with angular velocity and its conversion from rpm to radians per second
  • Knowledge of trigonometric functions, specifically tangent for angle calculations
  • Basic principles of physics related to rotational motion
NEXT STEPS
  • Calculate angular velocity from frequency using the formula ω = 2πf/60
  • Learn how to derive the radius from the centripetal acceleration equation
  • Explore the effects of tube angle on acceleration distribution in centrifugation
  • Investigate the implications of varying centrifuge speeds on blood component separation
USEFUL FOR

Students in physics or biomedical engineering, laboratory technicians involved in blood analysis, and professionals working with centrifugation techniques for plasma separation.

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Homework Statement


Human blood contains plasma, platelets, and blood cells. To separate the plasma from other components, centrifugation is used. Effective centrifugation requires subjecting blood to an acceleration of 2000g or more. In this situation, assume that blood is contained in test tubes of length L = 14.3 cm that are full of blood. These tubes ride in the centrifuge tilted at an angle of 45.0° above the horizontal (see figure below)

(a) What is the distance of a sample of blood from the rotation axis of a centrifuge rotating at a frequency f = 3480 rpm, if it has an acceleration of 2000g?
cm

(b) If the blood at the center of the tubes revolves around the rotation axis at the radius calculated in Part (a), calculate the accelerations experienced by the blood at each end of the test tube. Express all accelerations as multiples of g.
minimum acceleration g
maximum acceleration g



Homework Equations


Is this the correct equation for problem a:

atan=ac*r


The Attempt at a Solution



2000g=((2*pi*r)/(1/58))2*r
.015=r3
r=.25 cm
 
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I think the formula is a = v²/r, where v = 2πrN/T
N is the 3480 turns and T the 60 seconds.
When substituting the v equation into the a one, one of the r's will cancel out - you will not get an r cubed. The 2π will be squared.
 

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