Can I Determine Unknown Sample Concentration Using Absorption Spectroscopy?

AI Thread Summary
To determine the concentration of a sample using the USB4000 spectrometer and Beer-Lambert law, the absorbance value (A) and path length (l) are essential. It is suggested to take at least two absorbance measurements with different path lengths to improve accuracy. Using a cuvette with a rectangular cross-section allows for this by rotating it 90 degrees for the second measurement. This approach helps in calculating the unknown concentration effectively. Accurate measurements and proper setup are crucial for reliable results.
sukanyasv
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Hi

I'm trying to measure the concentration of a sample dissolved in water. I'm using the spectrometer USB4000 which directly gives me the absorbance value. Using beer-lambert law is there a way i can determine the unknown sample concentration from this measurement? I have the values 'A' and 'l' for the law. can someone throw some light on this??
 
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I think you need to have at elast two measurements of the absorbance with different thicknesses. There are cells for this- a rectangular cross section, so you do two readings with the cuvette rotated 90 degrees.
 

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