Can Mass Spectrometry be a difficult technique?

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Mass spectrometry can present challenges, particularly when analyzing isotopes with significantly varying abundances, such as carbon isotopes C12, C13, and C14. The low abundance of C14, at 0.0000000001%, raises concerns about detection sensitivity and potential misattribution of isotopes, such as confusing C13 with C12. These issues can complicate the interpretation of mass spectra, as the presence of trace isotopes may be obscured by more abundant ones. Additionally, the precision of measurements can be affected by the overlapping signals from isotopes with similar mass-to-charge ratios. Overall, careful consideration and advanced techniques are necessary to accurately analyze samples with such low isotope abundances.
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I'm just wondering if there are any difficulties that must be overcome to use mass spectrometry. For example if you were to use it with the three isotopes of Carbon ( C12, C13, and C14) where C12 has an abundance of 99%, C13 1% and C14 0.0000000001%. Based on the abundances how would mass spectrometry be difficult? I'm just curious and I couldn't find anything on the internet. Thanks
 
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What kinds of problems are you thinking about? Misattributing a ^{13}C to ^{12}CH? How do you think that such low abundances would affect the spectrum?
 
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