The discussion centers on the relationship between wavelength, frequency, and perceived color when monochromatic light transitions between different media. It highlights that while wavelength decreases when light enters a denser medium, the frequency remains constant, suggesting that frequency is a more reliable measure of color. The conversation also explores how light behaves when passing through diffraction gratings, noting that the medium after the grating affects the path length more than the medium before it. Additionally, it raises the question of whether color perception changes based on the medium, despite the light's wavelength being altered. Ultimately, it concludes that the human eye detects color based on wavelength, but frequency determines photon energy and thus the interaction with retinal pigments.