Difference between opposite states

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t_r_theta_phi
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Let's say there are two 1/2 spin particles, one in state

1/√2 |up> + 1/√2 |down>

and the other in the state

- 1/√2 |up> - 1/√2 |down>

Both particles then have an equal chance of being measured to be in either the up or down states. Is there any physical difference between these or are they indistinguishable?
 
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You can take out a global phase factor of -1 or ( e^(i*pi) ) from the second one and return the first one you mentioned. (By which I simply mean you can take out a factor of -1). This phase factor does not effect something called the expectation values of the Hermitian operator, which means, that the possible outcomes of either system should be the same. Or in this case, these two vector spaces are the same.
 
A pure state is represented not by a vector but by a ray in Hilbert space or, equivalently, a projection operator ##\hat{P}_{\psi}=|\psi \rangle \langle \psi |## with a normalized vector ##|\psi \rangle##. The most general state is given by an arbitrary statistical operator, i.e., a positive semidefinite self-adjoint operator with trace 1.