In the discussion about dissolved oxygen (DO) availability in two identical containers with different water volumes, it is clarified that both containers initially have the same concentration of dissolved oxygen per liter. Therefore, the total amount of DO is greater in the container with more water, but the concentration remains constant across both. The fish utilize oxygen from a small volume of water at a time, making the total water volume less critical as long as the container is not too small. However, in smaller containers, the rate of DO replenishment from the atmosphere may not keep pace with the fish's consumption, potentially leading to quicker depletion of oxygen. Factors such as the surface area exposed to the atmosphere and water agitation influence the efficiency of oxygen replenishment. Ultimately, while both fish have access to the same initial DO concentration, the fish in smaller containers may face challenges sooner due to limited oxygen replenishment.