Electrophoresis kinetic friction or not?

Click For Summary
SUMMARY

Electrophoresis separates substances based on charge and size, with mobility influenced by viscosity and resistance. In this context, lower density particles exhibit reduced movement due to increased volume and resistance, contrary to expectations that they should travel further. The discussion highlights that the resistance in electrophoresis is primarily related to viscous effects, particularly in applications like Western Blots, where kinetic friction is not the primary factor affecting particle movement.

PREREQUISITES
  • Understanding of electrophoresis principles
  • Knowledge of viscosity and its effects on particle movement
  • Familiarity with Western Blot techniques
  • Basic concepts of charge and size in particle separation
NEXT STEPS
  • Research the role of viscosity in electrophoresis
  • Study the principles of Western Blotting in detail
  • Explore the relationship between particle charge and mobility
  • Investigate different types of electrophoresis techniques
USEFUL FOR

Researchers, biochemists, and laboratory technicians involved in electrophoresis and protein analysis, particularly those working with Western Blots and particle separation techniques.

sameeralord
Messages
659
Reaction score
3
Hello everyone,

In electrophoresis the least dense one moved less (other factors been the same for every particle). I'm assuming this is due to higher volume and more resistance but, doesn't electrophoresis measure kinetic friction, so the one with lowest density should move further. What kind of resistance does electrophoresis refer to. Thanks :smile:
 
Physics news on Phys.org
If you are talking about, for example, Western Blots, 'friction' is manifested in terms of viscosity- the process separates substances by charge and size, and the mobility of the charged (mobile) phase depends on viscous effects (which are related back to the charge and size).
 

Similar threads

Undergrad Why does resistance reduce current in whole circuit?
  • · Replies 38 ·
2
Replies
38
Views
6K
Undergrad Numerical calculations of a railgun yielded disappointing results
  • · Replies 4 ·
Replies
4
Views
2K
High School What are the rules for static and kinetic friction?
  • · Replies 3 ·
Replies
3
Views
5K
I rewrote the Friction chapter from Tipler & Mosca
  • · Replies 6 ·
Replies
6
Views
2K
Undergrad Same old question, I still don't get it: E=1/2mv^2 - more E with V?
  • · Replies 41 ·
2
Replies
41
Views
4K
Conservation of Mechanical Energy and Kinetic Friction
  • · Replies 3 ·
Replies
3
Views
2K
What Is the Effect of Friction on the Height of a Sphere Rolling Down a Ramp?
  • · Replies 2 ·
Replies
2
Views
3K
High School Understanding Coefficient of Friction: Answers to Common Questions
  • · Replies 1 ·
Replies
1
Views
2K
Conservation of energy interpretation of percentage conserved
  • · Replies 20 ·
Replies
20
Views
3K
Questions on DNA recombinant DNA technology?
  • · Replies 3 ·
Replies
3
Views
7K