Chemistry Experimental Molecular Weight higher than Literature Value

AI Thread Summary
The calculated molecular weight of alkaline phosphatase is reported as 100,000 g/mol, exceeding the literature value of 89,000 g/mol. Possible reasons for this discrepancy include the presence of EDTA, which could affect the enzyme's weight. Questions were raised about the purification method, particularly whether gel electrophoresis was used and if the product was fully dialyzed. The lack of detail regarding the molecular weight determination method limits further analysis. Understanding the purification process and potential contaminants is crucial for resolving the observed difference.
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Homework Statement


I just purified alkaline phosphatase, and my calculated molecular weight is 100000 g/mol, while literature value is 89000 g/mol. According to how others in my lab did calculations, my calculations are performed correctly, so I am left to explain why my purification is higher than expected.

Homework Equations


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The Attempt at a Solution


I was thinking that since alkaline phosphatase can be inhibited by EDTA, my "purified" enzyme contains some EDTA, making the molecular weight higher.
 
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Did you purify it by gel electrophoresis? What was the supporting electrolyte? Did you completely dialyze your product?
 
You do not give any indication of what method used for getting the MW so what can anyone say?
 
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