MATLAB In these images,how can I remove the backgrnd noise in matlab?

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The discussion centers on isolating a cell from a background with similar intensity levels, making it difficult to differentiate between the two. The user has applied a median filter (medfilt2) twice, resulting in an image where the cell is no longer the brightest spot. This raises the question of whether to use a different filtering method or to adjust the criteria for locating the cell. Suggestions are sought for effective filtering techniques or alternative approaches to enhance the visibility of the cell against the background.
Urmi Roy
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I have to isolate the cell(seen as a bright blob) in the attached images from the background the zig-zag.

The problem is essentially that the intensity/brightness of the cell is almost equal to its backgraound, so I can't just subtract frames!
 

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After filtering the images with medfilt2 twice, I get the following image...
http://imgur.com/D60kG

However, the cell is no longer the brightest spot here, so do I go for some other filter or does the criterion on the basis of which I locate the cell change?

Please suggest.
 

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