Comparing Structural Determination Methods for Methodcomparosin (MC)

[carus88]

Homework Statement

' In a recent publication in the Journal of unusual Protein Structures, the authors reported the structure of the protein methodcomparosin (MC). They compared different methods for structural determination, as detailed below, and reported that the same structure was obtained with each method.

• 
  method 1: X-ray crystalography (usind synchrotron radiation with a wavelength of 0.35 angstrom meters
  method 2: 2D-NOSEY-NMR spectroscopy
  method 3: electron diffraction (using 40eV electrons)
  method 4: neutron diffraction (using neutrons with a speed of 9500 m/s)

In the publication, the authors claim to have obtained the structure of MC with a resolution of 0.5 angstrom meters or better for all methods. For each of the methods which they used state if this can be true and justify your answer.

Homework Equations

ke=0.5m(v^2) E=hv ke=(p^2)/2m

(DON'T KNOW IF THESE ARE RIGHT NONE ARE SUPPLIED)

The Attempt at a Solution

I'm not sure how to start I'm guessing i need to work out the respective resolution wavelengths for each method?
Just some guidance to get me started would be very helpful.

Thank you for your time.

 

[Jhero]

Thank you for bringing this publication to our attention. it is important to critically evaluate the methods and results presented in any publication. In this case, the authors claim to have obtained the structure of MC with a resolution of 0.5 angstrom meters or better for all methods. Let's take a closer look at each method and see if this claim is feasible.

Method 1: X-ray crystallography is a widely used method for determining protein structures. The resolution of this method is dependent on the wavelength of the X-rays used. In this case, the authors used synchrotron radiation with a wavelength of 0.35 angstrom meters. With this wavelength, a resolution of 0.5 angstrom meters or better is certainly possible.

Method 2: 2D-NOSEY-NMR spectroscopy is a technique that uses nuclear magnetic resonance to determine the structure of molecules. The resolution of this method is dependent on the strength of the magnetic field used. The authors have not specified the strength of the magnetic field used, but assuming it is strong enough, a resolution of 0.5 angstrom meters or better is possible.

Method 3: Electron diffraction is a technique that uses high-energy electrons to determine the structure of molecules. The resolution of this method is dependent on the energy of the electrons used. In this case, the authors used 40eV electrons, which may not be enough to achieve a resolution of 0.5 angstrom meters or better.

Method 4: Neutron diffraction is a technique that uses neutrons to determine the structure of molecules. The resolution of this method is dependent on the speed of the neutrons used. In this case, the authors used neutrons with a speed of 9500 m/s, which may not be enough to achieve a resolution of 0.5 angstrom meters or better.

In conclusion, while it is possible to obtain a resolution of 0.5 angstrom meters or better with methods 1 and 2, it may not be possible with methods 3 and 4. It is important for the authors to provide more information about the specific parameters and conditions used for each method in order to justify their claim. As scientists, it is our responsibility to critically evaluate and question the results presented in publications. I hope this helps guide your understanding of the methods used in this study.