Make 1L Culture Media: 10X Liquid Salt & FBS

AI Thread Summary
To prepare 1 liter of culture media, a 10X liquid salt solution must be diluted to 1X, requiring 0.1 liters of the 10X solution. Additionally, to achieve a final concentration of 10% fetal bovine serum, 0.1 liters of 100% serum is needed. The remaining volume should be filled with water, which totals 0.8 liters. The calculations confirm the volumes needed for each component, ensuring proper dilution. This method effectively combines the necessary ingredients to create the desired culture media.
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Homework Statement


You need to prepare medium for your culture cells. Your liquid salt solution is 10X concentration, you need to dilute to 1X for use. You need to add 100% fetal bovine serum for a final concentration of 10%. How would you make up 1 liter of this culture media using water as your solvent?

Homework Equations


possibly c1v1=c2v2

The Attempt at a Solution


Liquid Salt
(10)(v1)=(1)(1L)
v1=0.1L

Fetal Bovine Serum
(1)(v1)= (.1)(1L)
v1=.1L

1 Liter - 0.1L - 0.1L = 0.8L of Water

Add 0.1L of the 10X liquid salt to 0.1L of the 100% serum, dilute with water to 1 L.
 
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So how is this a molarity calculation?
 
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