Methods fo Complementary base pairing properties of DNA

[jena]

Hi,

My question asks to:

List the methods that use the complementary base pairing properties of DNA (hint; any methods that use DNA-DNA or DNA-RNA hybridization); include those that we covered in Recombinant DNA Technology and Genomics. You can include a few extra ones from the book.


My Answer

The methods that used complementary base pairing properties of DNA include Polymerase Chain Reaction(PCR), Gene Cloning, Sanger Method, Site directed Mutagenesis, and DNA polymorphisms.

Is this correct

Thank You

 

[iansmith]

The method you listed are ok but DNA polymorphisms is not a method; however you can id DNA polymorphisms using certain methods. Hint: finger.

Also most of the techniques you listed are based on the same "PCR" idea. There is more to it.

With this statements "DNA-DNA or DNA-RNA hybridization" in mind, there a least 3 major methods/techniques that you are missing. One is an older method that was used to measure the "relatedness" among organism, at least in bacteria. The other two are basicly the same as a method used in Protein science. Hint: Western.

 

[jena]

Hi,

Are Northern and Southern blotting okay, as well as DNA fingerprinting/typing, Maxam-Gilbert sequencing, and pyrosequencing.

Thank You

 

[iansmith]

You should read on the Maxam-gilbert sequencing method. You will get an answer. In terms of hybridization, the Sanger method is the opposite of the Maxam-Gilbert method.
http://www.bookrags.com/sciences/genetics/sequencing-dna-gen-04.html

Northern and southern blots (the N and S should not be capitalized because it is not named after a person), DNA fingerprinting/typing and pyrosequencing are okay.

You should also look into global gene expression profiles methods.

 

[pattylou]

Southern was the original blot method, and *was* named after a person. The other two weren't named after people.

Southerm EM 1975. JMB, 98:503-517.

 

[iansmith]

Southern was the original blot method, and *was* named after a person. The other two weren't named after people.

Southerm EM 1975. JMB, 98:503-517.

Hah cranky! I should of remember that! it was a question during somebodies defence.

 

[jena]

Hi,

Can viruses be counted in base pairs or are they only counted in nucleotides? If they are only counted in nucleotides is there any other way to convert them to base pairs?

Thank You

 

[iansmith]

nucleotides and base pairs are basicly the same. The only difference is that base pair refers to two nucleotides join together by an hydrogen bound.

Viruses can be either RNA or DNA, and the DNA or the RNA is double or single stranded.

It would be good if could rephrase your questions with better terms.