MilliQ water and RO water

  • Thread starter kkbniist
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In summary, the conversation suggests that using normal RO water with a lower conductivity may be a suitable replacement for MilliQ water in preparing buffer for molecular work. However, it is important to ensure that this does not violate any protocols and to clearly state this change in the reporting. It is also recommended to keep replacement parts on hand for critical equipment such as the MilliQ system to avoid delays in work.
  • #1
kkbniist
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Hi
can we replace MilliQ water (18.2MΩ) with normal RO water (22KΩ) ?
The application is for preparing buffer for a molecular work.

thanks in advance
kkb
 
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  • #2
I guess so, as long as it does not violate your protocols. In other words, you have to stipulate in your reporting that you did this. Since the amount of information you gave is sparse my answer is equally vague.

Is this is for a lab quiz/project in college? Do the written procedures state that 18.2m Ohms is required? Is some entity paying you to do this work, a client? Generally using milliQ water is done to introduce required repeatability and "bona fides" of an analysis. Only you know what is going on.
 
  • #3
thanks jim mcnamara
In fact we have a MilliQ system in our lab. But suddenly its membrane needs replacement.
The procurement of the membrane takes some time.
We have many routine molecular biology works that recommends MilliQ water.
Meanwhile, we have an RO system in our lab with.
So I thought of using the RO water which has a much lower conductivity also.

thanks
 
  • #4
Sounds like you should keep membranes on hand. You said "suggests" MilliQ water. Most lab protocols are not usually worded that way ("suggested"), hmm. If something is "suggested" that implies it is not required, so RO should be fine. Just report it that way.

If something like an RO setup or a MilliQ, is in the critical path of performing work, good practice is considered to be: 'have replacement parts on hand'. Especially for components that regularly need it.
 
  • #5


I would not recommend replacing MilliQ water with normal RO water for preparing buffers for molecular work. MilliQ water, with a conductivity of 18.2MΩ, is considered ultrapure and is typically used for sensitive experiments that require high purity and low levels of contaminants. RO water, with a conductivity of 22KΩ, may contain higher levels of impurities that could potentially affect the results of your molecular work. Additionally, MilliQ water is specifically designed for molecular work, while RO water may not have the same level of quality control and may not be suitable for this application. It is important to use the appropriate water for your experiments to ensure accurate and reliable results.
 

What is the difference between MilliQ water and RO water?

MilliQ water and RO water are both forms of purified water, but they differ in the process by which they are produced. MilliQ water is produced through a process called deionization, which removes charged particles from water using ion exchange resins. RO water, on the other hand, is produced through a process called reverse osmosis, which uses a semipermeable membrane to remove impurities from water. This results in a difference in the level of purity between the two types of water, with MilliQ water being slightly purer than RO water.

Is MilliQ water safe for consumption?

Yes, MilliQ water is safe for consumption. As mentioned before, it undergoes a process of deionization which removes impurities and contaminants from the water. This results in a high level of purity, making it safe for human consumption. However, it is important to note that MilliQ water should not be used as a sole source of hydration as it lacks essential minerals that our bodies need.

Can MilliQ water be used in scientific experiments?

Yes, MilliQ water is commonly used in scientific experiments due to its high level of purity. This ensures that the water will not introduce any contaminants or interfere with the results of the experiment. It is also commonly used in laboratories for the preparation of various solutions and reagents.

How is the quality of MilliQ water and RO water maintained?

The quality of MilliQ water and RO water is maintained through regular testing and monitoring. Production facilities have strict quality control measures in place to ensure that the water meets the required standards of purity. Additionally, the water is often treated with UV light or ozone to prevent bacterial growth and maintain its purity.

Can MilliQ water and RO water be used interchangeably?

In most cases, MilliQ water and RO water can be used interchangeably. However, due to the slight difference in purity between the two, there may be some instances where one may be preferred over the other. For example, MilliQ water is often preferred for sensitive experiments or for preparing solutions that require a high level of purity. It is best to consult with a scientist or lab technician to determine which type of water is most suitable for a specific use.

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