- Hi there. I'm a PhD student learning how to interpret raw microarray data. I have selected a miRNA expression profile of mice liver tissue, of three groups: Control, High-fat diet(HFD) and HFD+drug.
Main Question or Discussion Point
Following are the two different types of bar-digram that were observed. My questions are:
1. What do bars hanging below 0 mean in the first dig. ?
2. What do bars shooting from -6 mean, also why are they below 0?
3. Like a normal gene (non-ncRNA gene) expression profile where the expression level is measured by the logFC value, is F the parameter that is analogous to logFC here? Could you shed some light as how F works?