Need help on protein alignment quick!

  • Thread starter megga124
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I am trying to do this protein alignment assignment for my Biotech class and I'm having trouble interpreting the results. I used the Blast program at www.ncbi.nlm.nih.gov/blast/bl2seq/bl2.html[/URL] and have to answer the following questions: What are "Query" and "Sbjct"?, What do the letters of the middle row signify?, What do the "+" signs of the middle row signify?, What does "conservation" mean when referring to proteins?, and Why does actin's ability to bind many other proteins cause its sequence to be highly conserved?

I don't really have a clue as to the answers to these questions, so any help at all would be grately appreciated! The project is due tomorrow (Thursday) at 1pm.

Thanks.
 
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