Optical Tweezers: Preparing Samples for RBC Trapping

[henil]

I am currently working on project for trapping RBC's using optical tweezers but i think i am making a mistake in preparing the sample. Can anyone provide an appropriate sample preparing method ?

 

[berkeman]

I am currently working on project for trapping RBC's using optical tweezers but i think i am making a mistake in preparing the sample. Can anyone provide an appropriate sample preparing method ?

I did a Google search on optical tweezers for trapping red blood cells, and got lots of good hits. This was the first article on the list:

http://www.nature.com/ncomms/journal/v4/n4/full/ncomms2786.html

Maybe try doing that search, to see if you find what you need.

 

[Andy Resnick]

I am currently working on project for trapping RBC's using optical tweezers but i think i am making a mistake in preparing the sample. Can anyone provide an appropriate sample preparing method ?

Does your trap work on microspheres? Do you have a dilute suspension of RBCs?

 

[henil]

Does your trap work on microspheres? Do you have a dilute suspension of RBCs?

yes sir i do have worked on microspheres and i do have diluted blood with 1/2ml PBS, 1/2ml distilled DI water and a drop of blood. also the RBCs are not flipping .
i have tried changing the power supplied

 

[henil]

I did a Google search on optical tweezers for trapping red blood cells, and got lots of good hits. This was the first article on the list:

http://www.nature.com/ncomms/journal/v4/n4/full/ncomms2786.html

Maybe try doing that search, to see if you find what you need.

okay sir thank you.