Optical Tweezers: Preparing Samples for RBC Trapping

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Discussion Overview

The discussion revolves around the preparation of samples for trapping red blood cells (RBCs) using optical tweezers. Participants are seeking methods and troubleshooting tips related to sample preparation and the effectiveness of their trapping setups.

Discussion Character

  • Exploratory, Technical explanation, Debate/contested, Homework-related

Main Points Raised

  • One participant expresses concern about potentially making a mistake in preparing samples for trapping RBCs and requests appropriate methods.
  • Another participant suggests conducting a Google search for articles on optical tweezers and shares a specific article link as a potential resource.
  • A participant questions whether the optical trap works on microspheres and inquires about the dilution of the RBC suspension.
  • A participant confirms they have worked with microspheres and describes their sample preparation, including the use of PBS and distilled water, but notes issues with the RBCs not flipping.
  • One participant mentions trying to change the power supplied to the optical tweezers as part of their troubleshooting process.

Areas of Agreement / Disagreement

Participants do not appear to reach a consensus on the best sample preparation methods, and multiple viewpoints and suggestions are presented without resolution.

Contextual Notes

There are limitations regarding the specific conditions under which the optical tweezers operate effectively, as well as the assumptions about the sample preparation that have not been fully explored or clarified.

Who May Find This Useful

Researchers and students working on optical trapping techniques, particularly those focused on biological samples like red blood cells.

henil
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I am currently working on project for trapping RBC's using optical tweezers but i think i am making a mistake in preparing the sample. Can anyone provide an appropriate sample preparing method ?
 
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henil said:
I am currently working on project for trapping RBC's using optical tweezers but i think i am making a mistake in preparing the sample. Can anyone provide an appropriate sample preparing method ?
I did a Google search on optical tweezers for trapping red blood cells, and got lots of good hits. This was the first article on the list:

http://www.nature.com/ncomms/journal/v4/n4/full/ncomms2786.html

Maybe try doing that search, to see if you find what you need. :smile:
 
henil said:
I am currently working on project for trapping RBC's using optical tweezers but i think i am making a mistake in preparing the sample. Can anyone provide an appropriate sample preparing method ?

Does your trap work on microspheres? Do you have a dilute suspension of RBCs?
 
Andy Resnick said:
Does your trap work on microspheres? Do you have a dilute suspension of RBCs?
yes sir i do have worked on microspheres and i do have diluted blood with 1/2ml PBS, 1/2ml distilled DI water and a drop of blood. also the RBCs are not flipping .
i have tried changing the power supplied
 

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