When removing 100 mL from a 500 mL bottle of vinegar containing 5% acetic acid, the concentration of acetic acid remains at 5% in both the removed portion and the remaining solution. Concentrations are consistent in a well-mixed solution, meaning that regardless of the volume taken out, the percentage of acetic acid does not change. This principle can be illustrated with a population analogy, where if 50% of a country's population is female, the same percentage applies to any subset of that population, such as a city.