Using an absorbance maximum for assays is crucial because it ensures higher reliability and precision in quantitative analysis, particularly in UV-Vis spectroscopy. The maximum absorbance correlates with the highest absorptivity, which enhances the signal-to-noise ratio, making detection more accurate. In contrast, using an absorbance minimum could lead to compromised data quality and less reliable results, as the signal may be too weak and more susceptible to noise. Broader peaks at maximum absorbance also minimize the impact of peak position shifts on intensity values. Overall, selecting the maximum absorbance is essential for effective and precise analytical measurements.
