Different diffraction gratings with varying line spacings affect the observation of emission lines from gases like Hydrogen and Neon by altering the dispersion of colors. Closer line spacing results in a greater spread of colors, allowing for better resolution of closely spaced spectral lines, such as the sodium doublet. Experiments indicate that using gratings with more lines per millimeter can reveal additional colors, enhancing visibility. However, it is a misconception that tighter spacing prevents certain wavelengths from passing through. Overall, the choice of diffraction grating significantly influences the spectral analysis of light emissions.