The relationship F = -dV/dx describes how force relates to the change in potential energy per distance, with V representing electric potential difference. The electric field E can be expressed as E = -dΦ/dx, leading to the conclusion that force F equals -q(dΦ/dx) or F = -dV/dx when considering charge q. In three dimensions, this relationship is generalized using the gradient operator. The discussion also touches on the interpretation of potential in quantum mechanics, questioning the implications of a particle having V=0. Understanding potential in this context raises questions about a particle's behavior and whether it implies perpetual motion.