Use of statistics in experiment

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Astudious
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I have seen that "the best estimate for the random error σ(X) in a single measurement is given by

σ(X)2 ≈ 1/(n-1) * ∑((xi-μ)2) where the sum is over all i"

I have two questions about this: firstly, how can this pertain to a "single measurement" if it requires the data from multiple measurements (x1, x2, x3, ... xi)? Secondly, this seems to correspond to the sample variance - wouldn't it be a more accurate estimate of the value of X's random error to convert to the variance of the population of X as a whole?
 
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If you want to use several data points that you already have to predict what will happen for a new or unknown single data point, that is the equation you should use.

PS, The correct equation uses the sample average of the existing data in place of μ. If some how you know μ, you can use it, but divide by n rather than n-1.