What are ions of molecules interacting with in vivo

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Charged amino acids in physiological conditions can interact with various oppositely charged entities, but the specifics of these interactions remain unclear. The discussion draws parallels to organic chemistry, where acid-base reactions form salts, questioning what charged amino acids form salts with in vivo. It highlights that, similar to how sodium chloride dissociates in solution, most ions within cells exist as separate entities rather than in stable associations. However, exceptions exist, such as magnesium ions associating with nucleotide triphosphates like ATP or specific proteins binding ions. The equilibrium conditions in the cytosol favor the ionic forms of potassium and amino acids, suggesting that while charged amino acids may not need to form stable salts, their interactions with other charged species are influenced by these equilibrium dynamics.
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For example:

amino acids that are floating around, some with charge at physiological pH. My question is what exactly do the charged amino acids form a salt with? other amino acids? sugars? fatty acids? whatever happens to have an opposite charge and is close?

A rephrasing: I am thinking about how how in o chem acid base reactions we added sodium hydroxide to a solution of benzoic acid to create the conjugate base of benzoic acid. In the case the benzoic acid forms a salt with the sodium, so what do the amino acids form salts with in vivo?
 
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Most salts in solution exist as separated ions. If you dissolve table salt in water, you have Na+ ions and Cl- ions in solution, which are not stably associated with each other. In the same way, most of the ions inside the cell are not stably associated with counter ions.

There are, of course, exceptions to this rule. For example, Mg2+ fairly stably associates with nucleotide triphosphates like ATP, and various proteins or other biomolecules often have specific charged sites that will stably bind ions from solution.
 
vande060 said:
For example:

amino acids that are floating around, some with charge at physiological pH. My question is what exactly do the charged amino acids form a salt with?

Do they need to? Equilibrium conditions favour the ionic forms of ions like potassium and the various amino acids in the cytosol.
 
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