Persistence length is a key concept in understanding the flexibility of polymers, such as DNA and microtubules. It measures the distance over which a polymer behaves like a rigid rod versus a flexible noodle. At distances shorter than the persistence length, the polymer maintains a consistent direction, indicating rigidity. Conversely, at distances longer than the persistence length, the polymer's direction becomes uncorrelated, demonstrating increased flexibility. The persistence length is characterized by an exponential decay in the correlation of tangent angles as distance increases. This concept is crucial for analyzing the mechanical properties of long, flexible structures in biological systems. Additionally, challenges in calculating persistence length using correlation functions arise when dealing with negative values, which complicates fitting the exponential model.