What Sub-Structure Does a 369.3 Mass Indicate in Cholesterol LCMS Analysis?

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The discussion centers on interpreting a mass of 369.3 observed in LCMS analysis, which is related to cholesterol. Participants clarify that the molecular mass of cholesterol is 387.3, and the discrepancy arises because the LCMS result reflects a mass that is 18 units less due to the loss of a water molecule. Understanding this relationship helps in determining the correct sub-structure associated with the observed mass. The conversation emphasizes the importance of recognizing mass shifts in LCMS results for accurate structural identification. Overall, the analysis highlights the complexities of interpreting mass spectrometry data in biochemical contexts.
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Homework Statement


Our assignment was to determine the structure, or sub-structure of our mass observed in the LCMS result. The number we got was 369.3. I am not really sure what to do with this at all. She gave us the structure of cholesterol, but I'm not sure where to add things on...
 
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cdoss said:

Homework Statement


Our assignment was to determine the structure, or sub-structure of our mass observed in the LCMS result. The number we got was 369.3. I am not really sure what to do with this at all. She gave us the structure of cholesterol, but I'm not sure where to add things on...

Add things on? Molecular mass of cholesterol is ... what?
 
387.3. I think I actually understand it now. because the LCMS will show the mass +1 and then the bond with water was broken, which caused the weight to be 18 less than what we expected.?
 
cdoss said:
387.3. I think I actually understand it now. because the LCMS will show the mass +1 and then the bond with water was broken, which caused the weight to be 18 less than what we expected.?

yeah,,, agree,,,!:cool::cool::cool::cool::cool::cool::cool:
 
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