Why did my Biuret's test on eggwhite result in a brown color?

  • Thread starter Thread starter Fluff
  • Start date Start date
  • Tags Tags
    Test
AI Thread Summary
The Biuret test for proteins resulted in a brown color instead of the expected blue, which indicates a potential issue with the test conditions. The discussion highlights that the presence of reducing sugars in the egg white could lead to unexpected reactions, possibly contributing to the brown color. Concerns about the pH level and copper concentration were raised, suggesting they might affect the outcome of the test. The user expressed frustration over not being able to identify the cause of the brown color and the potential implications for their report. Overall, the conversation revolves around troubleshooting the Biuret test results and understanding the chemistry involved.
Fluff
Messages
9
Reaction score
0
I can't seem to figure out why the test came out as BROWN. I've been researching for HOURS and I can't find out why. I'm not asking any of you to give me an answer, but PLEASE throw me a hint. PLEASE. I heated the egg to 37C by the way.
 
Physics news on Phys.org
Did you add too much CuSO4?
 
Biuret is a test for protein. The copper(II) is reduced to copper(I) in the presence of protein and it complexes the nitrogen groups, turning violet at high pH.

Benedict's test is a test for reducing sugars. It contains copper(II) that reacts with the reducing sugar to produce a brick red ppt of copper(I) oxide.

What might happen if the pH were too high in the Biuret test or if the copper concentration were too high? What might happen if the protein test sample also contained reducing sugars?
 
I didn't add too much CuSO4 -_- there was an amount we were supposed to put. =P

Yes. I knew that about the Biuret's but the result for negative was supposed to be blue =S And it was BROWN. So yeah. It buffled me. So if eggwhite had a reducing sugar, would it have a brickred precipitate too? And I did the report but I just moved on quickly so the teacher wouldn't have enough time to ask what caused the brown instead of blue -_-

I slipped away but I focused to the brown thing that I didn't read the other questions properly so I answered wrong. Bad days.

Haha, sorry if I just pop in here when I need something. -_- Thank you for the hint though haha.
 
Thread 'Confusion regarding a chemical kinetics problem'
TL;DR Summary: cannot find out error in solution proposed. [![question with rate laws][1]][1] Now the rate law for the reaction (i.e reaction rate) can be written as: $$ R= k[N_2O_5] $$ my main question is, WHAT is this reaction equal to? what I mean here is, whether $$k[N_2O_5]= -d[N_2O_5]/dt$$ or is it $$k[N_2O_5]= -1/2 \frac{d}{dt} [N_2O_5] $$ ? The latter seems to be more apt, as the reaction rate must be -1/2 (disappearance rate of N2O5), which adheres to the stoichiometry of the...
Back
Top