The greater the friction (and viscosity) the greater the resistance (opposition) to motion. As friction increases, the pressure drop must increase, and more work will have to be done on a fluid for a given flow rate.
The sign on the differentials depends on the coordinate system (reference) or orientation used, e.g. is the coordinate system (in a pipe flow) measured from the center of the flow outward, or from the pipe wall inward.
What does du/dx < 0 mean? Simply as the distance/position increases, then u is decreasing. If du/dx increases, then u is increasing, i.e. the fluid is accelerating with x.
dP/dx < 0 means a pressure drop as x increases, or dP/dx > 0 means pressure increases with x. Then one has to look at what causes the Pressure to increase or decrease.