Wool is used at the bottom of the resonance tube to prevent damage to the glass when the tube rests on a solid surface, minimizing the risk of breakage during experiments. The padding absorbs impact and protects the apparatus during the resonance experiment, which involves precise sound measurements. Participants in the discussion emphasize the importance of clear communication regarding experimental setups, especially when different educational backgrounds may lead to confusion. The practical procedure involves filling the cylinder with water, adding wool, and then using a tuning fork to measure sound resonance. Overall, the wool serves a crucial safety function in the experiment.