Why Use Restriction Enzymes and Gel Electrophoresis in Molecular Biology?

  • Thread starter Thread starter solijigar
  • Start date Start date
  • Tags Tags
    Dna Lab
AI Thread Summary
Restriction endonucleases in prokaryotes serve to protect their DNA from foreign genetic material by recognizing and cutting specific sequences. Using purified DNA samples in restriction digest and gel electrophoresis is crucial because it ensures accurate results, as homogenized tissue may contain contaminants that interfere with the analysis. The combination of restriction enzymes and gel electrophoresis is fundamental in molecular biology for DNA manipulation and analysis, allowing researchers to visualize and separate DNA fragments based on size. The discussion highlights the importance of understanding these techniques and encourages users to refer to textbooks or lecture notes for foundational knowledge. Overall, mastering these methods is essential for effective molecular biology research.
solijigar
Messages
2
Reaction score
0
[SOLVED] BiOchem lab DNA electrophoresis

1) what is the the purpose of restriction endonucleases in prokaryotes? how do these organisms protect their DNA against the effects of their own restriction enzyme?


2)why is it important to use purified Dna samplesin restriction digest and gel electrophoresis? Rather then just using homogenized tissue?


3) why we use restriction enzyme and gel electophoresis in molecular biology?
 
Physics news on Phys.org
Have you tried to Google this. I'm sure you can find it on Wikipedia.
 
The answers to these sorts of questions really should just be in your textbook or lecture notes. Once you've found them, if there's something you need explained further or clarified, post the answer you get and your more specific question about it, and we can then help.
 
well thanks any way I already have found the answer.I were just streesd out and weren't able to focus...
 
Thread 'Confusion regarding a chemical kinetics problem'
TL;DR Summary: cannot find out error in solution proposed. [![question with rate laws][1]][1] Now the rate law for the reaction (i.e reaction rate) can be written as: $$ R= k[N_2O_5] $$ my main question is, WHAT is this reaction equal to? what I mean here is, whether $$k[N_2O_5]= -d[N_2O_5]/dt$$ or is it $$k[N_2O_5]= -1/2 \frac{d}{dt} [N_2O_5] $$ ? The latter seems to be more apt, as the reaction rate must be -1/2 (disappearance rate of N2O5), which adheres to the stoichiometry of the...
I don't get how to argue it. i can prove: evolution is the ability to adapt, whether it's progression or regression from some point of view, so if evolution is not constant then animal generations couldn`t stay alive for a big amount of time because when climate is changing this generations die. but they dont. so evolution is constant. but its not an argument, right? how to fing arguments when i only prove it.. analytically, i guess it called that (this is indirectly related to biology, im...
Back
Top