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Graduate Calculate persistence length from force extension data of a single DNA
then unfortunately the plot was for a different force range, however in case the intercept is 1.4, the persistence length would be ~2nm (still much too low). -
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Chemistry Calculate persistence length of a single dsDNA molecule
Hello Epenguin! Sorry, my mistake: it should be \big(1-\frac{x}{L_0}\Big)^{-2} instead of \big(1-\frac{x}{L_0}\Big) in the equation.- pen
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- Forum: Biology and Chemistry Homework Help
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Chemistry Calculate persistence length of a single dsDNA molecule
Hello! 1. The problem statement and all variables AIM: Calculating persistence length P of a single dsDNA molecule from a data set of force F (to the molecule) vs. extension x measurements. Experimental background: pN forces were applied to a single dsDNA molecule spanned between two...- pen
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- Length Molecule Optics
- Replies: 3
- Forum: Biology and Chemistry Homework Help
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Graduate Calculate persistence length from force extension data of a single DNA
Hello! From a data set of F-x measurements of a single dsDNA molecule I want to calculate the persistence length P . So I plotted \frac {1} {\sqrt{(F)}} vs. x and fitted these data points (linear). According to an interpolation formula the extension x of a worm like chain with contour... -
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Graduate Scattering force in optical trapping
thanks a lot! now I think: in the ray optics regime: The net force exerted on a microsphere by one ray is calculated considering the contribution of forces due to reflection and due to refraction, (depending on the incident angle of the ray). The net force can be decomposed into... -
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Graduate Scattering force in optical trapping
hello, reading about the theoretical background of optical trapping. I was wondering how the scattering force originates. Often it is written, that the scattering force is due to reflection, but also the central rays refracted by a dielectric object should contribute ? So the scattering force...